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KMID : 0379119800080030159
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Korean Journal of Mycology
1980 Volume.8 No. 3 p.159 ~ p.166
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Polyacrylamide Gel Electrophoresis of the Cell Proteins from Differentiating Aspergillus niger
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Abstract
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Aspergillus niger van Tieghem was cultured by the method of submerged and synchronized culture for the study of differentiation. Acid-phenol soluble cell proteins of the fungus were extracted from four stages during development. Those acid-phenal soluble proteins were separated by polyacrylamide gel electrophoresis to determine the protein patterns.
A new protein band was observed from the pre-sporulation body, color density of the stained protein bands in four tubes differed according to the differentiation stages.
The number of protein bands in 10% gels varied from 18 to 16, 17, and 19 according to the course of spore germination stage, conidiophore stage, phialide maturation stage and sporulation stage.
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